Cal activity data recording was provided to subjects, along with dataCal activity data recording was

Cal activity data recording was provided to subjects, along with data
Cal activity data recording was provided to subjects, along with data collection forms. An overview of study procedures was also provided. A maximal graded exercise test (GXT) was conducted using a treadmill while expired gases were collected via facemask and analyzed using a SensorMedics Vmax 229TM metabolic system for determination of maximal oxygen consumption (VO2max). The collection and analysis of expired gases provided further descriptive characteristics regarding participants’ aerobic power (e.g., VO2max). The test continued until volitional fatigue. Before and during the GXT, heart rate was continuously monitored via electrocardiograph (ECG) tracings using a SensorMedics Max1TM ECG unit. Expired oxygen and respiratory exchangePage 2 of(page number not for citation purposes)Lipids in Health and Disease 2009, 8:http://www.lipidworld.com/content/8/1/Table 1: Descriptive characteristics of 14 exercise-trained menVariable Age (yrs) Height (cm) Weight (kg) Body fat ( ) BMI (kg -2) Resting HR (bpm) Resting SBP(mmHg) Resting DBP(mmHg) VO2max (mL g-1 in-1) Years Aerobic Exercise Hours/wk Aerobic Exercise Years Anaerobic Exercise Hours/wk Anaerobic Exercise Data are mean ?SD.Value 25.5 ?4.8 174.4 ?6.2 73.4 ?7.7 10.0 ?3.6 24.1 ?1.6 56 ?8.7 112.1 ?9.8 68.6 ?7.2 47.0 ?3.5 3.9 ?3.6 4.4 ?3.8 6.3 ?3.8 5.3 ?3.Supplementation The study design involved a random order cross-over assignment to EPA/DHA and placebo in a double blind manner. A schematic of the study timeline is presented in Figure 1. The total daily dosage of EPA (2224 mg; MorEPA Mini; Minami Nutrition, Belgium) and DHA (2208 mg; MorDHA Mini; Minami Nutrition, Belgium) was provided in eight gel capsules taken twice per day (morning and evening) with meals. The placebo gel capsules (taken in the same quantities) consisted of soybean oil and were identical in appearance, texture, and taste to the EPA/DHA capsules.ratio data were continuously monitored via breath-bybreath samples. Blood pressure was monitored and the Borg scale of exertion was used to allow participants to indicate their level of perceived work. The highest 30 second average for VO2 was recorded as subjects’ VO2max. Five subjects who underwent testing failed to reach the 40 mL g-1 in-1 criteria. Therefore, these individuals were excluded from GS-5816 web participation, leaving us with a total of 15 subjects entering randomization.In both conditions, capsules were distributed to subjects by research assistants every two weeks in unlabeled boxes in amounts greater than needed for supplementation. Capsule counts upon box return allowed for estimation of compliance to intake.Acute exercise session Following each six week period of EPA/DHA or placebo intake, subjects reported to the lab in the morning to perform a submaximal exercise test. Subjects were asked to walk on a treadmill while carrying a weighted backpack (weight equal to 25 of body mass) for 60 minutes. The treadmill speed and grade were altered every five minutesFigure 1 Study timeline to investigate the effects of EPA/DHA on resting and exercise-induced inflammation and oxidative stress Study timeline to investigate the effects of EPA/DHA on resting and exercise-induced inflammation and oxidative stress.Page 3 of(page number not for citation purposes)http://www.lipidworld.com/content/8/1/Table 2: Exercise PubMed ID:http://www.ncbi.nlm.nih.gov/pubmed/27735993 data from 14 exercise-trained men during 60 minutes of treadmill walkingEnding Minute Speed (mph) Grade 5 10 15 20 25 30 35 40 45 50 553.53.53.53.53.53.53.53.53.53.0 12.