Otenoids contents are presented in Figure two and Additional file 2: Table S2. The highest

Otenoids contents are presented in Figure two and Additional file 2: Table S2. The highest chlorophyll content material of 38.76 g mL-1 and 4.eight g mL-1 total carotenoids were recorded on 8th day in sodium thiosulphate supplemented with tryptophan. The lipid productivity (mg g-1) in BBM and BBM+ST steadily enhanced from 16090 and 19-20 by the 12th day of Arachidic acid supplier incubation (Figure three). The lipid productivity was enhanced within the presence of tryptophan on the 4th day (390 mg g-1). Highest values have been observed in sodium thiosulphate + SC-58125 custom synthesis glucose supplemented culture,FFNgangkham et al. SpringerPlus 2012, 1:33 http:www.springerplus.comcontent11Page three ofALipids (g L-1)0.15 0.10 0.05 0.00 A1 A2 A3 A4 A5 A6 A7 TreatmentsDLipids (g L-1)0.25 0.20 0.15 0.ten 0.05 0.00 A1 A2 A3 A4 A5 A6 A7 TreatmentsBLipids (g L-1)0.30 0.25 0.20 0.15 0.10 0.05 0.00 A1 A2 A3 A4 A5 A6 A7 TreatmentsE0.30 Lipids (g L-1) 0.25 0.20 0.15 0.10 0.05 0.00 A1 A2 A3 A4 A5 A6 A7 TreatmentsC0.2 Lipids (g L-1) 0.15 0.1 0.05 0 A1 A2 A3 A4 A5 A6 A7 Remedies eat e tsFLipids (g L-1)0.25 0.20 0 15 0.15 0.ten 0.05 0.00 A1 A2 A3 A4 A5 A6 A7 TreatmentsFigure 1 Lipid accumulation in Chlorella sorokiniana MIC-G5 in the presence of reducing agent (Sodium thiosulphate and methyl viologen). (A-C) sodium thiosulphate on 4, eight, 12 d and (D-E) methyl viologen on 4, eight, 12d respectively. A denotes kind of treatment options; A1 (BBM+sucrose), A2 (BBM+fructose), A3 (BBM+sodium pyruvate), A4 (BBM+tryptophan), A5 (BBM+alanine), A6 (BBM+glucose), A7 (BBM, Control). Mean n = 3 replicates.upto 8th day. In niacin supplemented culture, no growth was recorded around the 4th day, but by the 8th and 12th day, 18.33 and 27.33 lipids have been recorded. The highest values have been recorded in 8th day cultures, with regards to lipid productivity ( DCW) in glucose supplemented remedy (39 or 390 mg g-1), and followed by sodium pyruvate (38 ) and Vitamin B12 (36 ).Upscaling of chosen promising substrates and impact on lipid productivity and qualityThe promising substrates – comprising tryptophan, glucose, sodium pyruvate and Vitamin B12 had been taken up further in 5 L flasks containing 2 L medium supplemented with sodium thiosulphate along with the chosen substrate. On 4th day, the highest values with regards to turbidity (Abs750 1.26), chlorophyll (eight.96 g mL-1) and carotenoids (1.53 g mL-1) were recorded in sodium thiosulphate + tryptophan therapy (Extra file 2: Table S3). Thehighest values of turbidity (Abs750 1.85), chlorophyll (16.six g mL-1) have been obtained in medium supplemented with sodium thiosulphate alone, but highest lipid content of 0.238 g L-1 was recorded in medium supplemented with glucose and sodium thiosulphate on 8th day of cultivation. Cultures supplemented with sodium thiosulphate + vitamin B12 recorded highest total carotenoids (1.46 g mL-1) d on 8th day of cultivation (Added file two: Table S4). In terms of lipid productivity ( DCW), medium supplemented with glucose and sodium thiosulphate recorded highest values of 33 and sodium pyruvate (31.33 ) on the 8th day, followed by tryptophan (30.33 ) around the 4th day (Figure four).Microscopic analysesFLight microscopic analyses showed that the cells appeared additional robust when grown in BBM with sodium thiosulphate and tryptophan for 4th day and in VitaminNgangkham et al. SpringerPlus 2012, 1:33 http:www.springerplus.comcontent11Page 4 ofAAbsorbance1.six 1.4 1.2 1.0 0.eight 06 0.six 0.four 0.two 0.0 T1 T2 T3 T4 T5 T6 T7 T8 T9 T10 T11 T12 T13 TTreatmentsBAbsorbance2.0 1.eight 1.6 1.four 1.two 1.0 0.eight 0.6 0.4 0.two 0.0 T1 T2.