Unique analytical columns were tested. The key element for this study was arsenic, so the analysis was optimized in all situations to separate the As(III), As(V), DMA, MMA, and AsB types. Right after locating the optimal conditions and separating all of the arsenic types, additional elements were added, and also the effectiveness from the analysis was checked. Out in the several forms of columns, the following were tested: PRP-X100 (250 mm four.1 mm, ten ), packing material form: PS-DVB/Trimethyl ammonium exchanger (Hamilton, Reno, NV, USA); PRPX-200 PEEK (250 mm 4.6 mm, 10 ), packing material type: PSDVB/Sulfonic Acid (Hamilton, USA); BioWAX (50 mm two.1 mm, five ), packing material variety: nonporous/dietyloamine (Agilent, Santa Clara, CA, USA); Supelco SAX (250 mm 4.6 mm, 5 ), packing material sort: silica gel, spherical particle platform/propyltrimethylammonium phase (Sigma ldrich, USA); Waters SAX (250 mm 4.6 mm, 5 ), packing material sort: silica-based quaternary ammonium bonded sorbent (Waters, Milford, MA, USA);Molecules 2021, 26,9 of-Dionex CS5A (250 mm 4 mm, 9 ), packing material kind: latex, DVB/Cation: Sulfonic Acid/Anion: Quaternary Ammonium (Thermo, Waltham, MA, USA); Dionex CG5A (50 mm four mm, 9 ), packing material form: latex, DVB/Cation: Sulfonic Acid/Anion: Quaternary Ammonium (Thermo, USA); Dionex AS22 (250 mm 4 mm, six ), packing material variety: DVB/Alkanol Quaternary Ammonium Ion (Thermo, USA); Dionex AG22 (250 mm 4 mm, six ), packing material kind: DVB/Alkanol Quaternary Ammonium Ion (Thermo, USA).Depending on the literature describing arsenic, antimony, chromium, and selenium Inositol nicotinate custom synthesis speciation analysis, diverse mobile phases were chosen for the experiment. From the a variety of kinds of mobile phases, ammonium carbonate (NH4 )two CO3 , ammonium bicarbonate NH4 HCO3 , nitric acid HNO3 , ammonium nitrate NH4 NO3 , and ethylenediamine tetraacetic acid EDTA had been examined (Sigma ldrich, USA). The top resolution final results had been
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