results, obtained by solution ion scan mode analysis, had been observed from the product ion

results, obtained by solution ion scan mode analysis, had been observed from the product ion spectra obtained immediately after the isolation of m/z 227.0 on Q1. This precursor ion is the solution ion spectra obtained soon after the isolation of m/z 227.0 on Q1. This precursor ion is likely represent the molecular ion ofionallegedalleged metabolite of 5, by de-nitration of probably to to represent the molecular an of an metabolite of 5, obtained obtained by denitration from the side chain. Figure 9a reports the comparison on the m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction before the incubation with compound 5 (dotted line) and following two hours’ incubation (continuous line). A chromatographic peak is CB1 Agonist Species evident in the retention time of 2.60 min only in theAntioxidants 2022, 11,12 ofthe side chain. Figure 9A reports the comparison of your m/z 227.0 chromatographic profiles obtained in the rat liver microsomal fraction prior to the incubation with compound 5 (dotted line) and right after two hours’ incubation (continuous line). A chromatographic peak is evident at the retention time of 2.60 min only inside the second profile, viz. following two hours’ incubation. The corresponding item ion spectrum, depicted in Figure 9B, exhibits the Antioxidants 2022, 10, x FOR PEER Overview 13 of 21 loss of consecutive fragments in the side chain and it is compatible using the supposed metabolite’s structure.Figure 9. (a) Superimposed mass chromatograms of thethe m/z 227.0 precursor ion, obtained from Figure 9. (A) Superimposed mass chromatograms of m/z 227.0 precursor ion, obtained in the rat liverliver microsomal fractiont at t0=(dotted line) and t = = two h (continuous line)incubation with all the rat microsomal fraction at = 0 (dotted line) and t two h (continuous line) incubation with compound 5. (b) Item ion spectrum of your chosen m/z 227.0 precursor, collected at two.60 min, compound 5. (B) Product ion spectrum with the chosen m/z 227.0 precursor, collected at 2.60 min, in the latter evaluation. from the latter evaluation.Analogue experiments had been Estrogen receptor Antagonist list executed around the rat liver microsomal fraction incubated Analogue experiments have been executed around the rat liver microsomal fraction incubated with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds for the molecular with compound 7. The m/z 288.0 precursor ion isolated on Q1 corresponds to the molecularalleged metabolite 7 metabolite 7 obtained right after single the side chain.in the side ion of the ion from the alleged obtained following single de-nitration of de-nitration Figure 10A chain. Figure 10a reports the m/z 288.0 chromatographic profiles obtained in the rat reports the comparison with the comparison with the m/z 288.0 chromatographic profiles obtained in the fraction before the incubationbefore compound 7 and right after two hours, liver microsomal rat liver microsomal fraction with the incubation with compound 7 and just after two This time, a chromatographic peak is evident in the retention time of three.78 respectively. hours, respectively. This time, a chromatographic peak is evident at the retention time of three.78 min only rat the profile in the rat liver microsomal fraction min only inside the profile in the in liver microsomal fraction collected soon after two hours’ collected immediately after two hours’ incubation. The ion spectrum, depicted ion Figure 10B, exhibits incubation. The corresponding product corresponding product in spectrum, depicted in Figure 10b, exhibits a fragmentation related to Figure 9b. The solution ion spe