Inhuman and experimental animals. [24,25] The possible antioxidant activity of Tamarind seeds

Inhuman and experimental animals. [24,25] The possible antioxidant activity of Tamarind seeds has currently been reported.[26,27] T. indica seed coat could play a vital part in chemical protection from oxidative harm by possessing endogenous antioxidants like phenolic compounds. The prospective antioxidant activity of Tamarind seeds have already been reported and the isolated antioxidant components are, 2-hydroxy-30, 40-dihydroxyacetophenone, methyl 3, 4- ihydroxybenzoate, three, 4-dihydroxyphenylacetate and epicatechin as well as oligomeric proanthocyanidins, phenolic compounds are procyanidin B2, epicatechin, procyanidin trimer, procyanidin tetramer, procyanidin pentamer, procyanidin hexamer, polymeric tannins, polymeric tannins.[28] It has been shown that aqueous extract of T. indica seeds have potent antidiabetic and antihyperlipidemic activities in STZ-induced diabetic male rat.[29] Here we studied the methanolic extract of C. cajan root (MCC) and methanolic extract of T. indica seeds (MTI) to evaluate their hypoglycemic activity. As antidiabetic activity is closely associated with antioxidant activity, within this communication, we have also assessed the antioxidant activity and total phenol content of those two plants.Materials AND METHODSCollection of plant partsRoots of the plant C. cajan and seeds of T. indica , had been collected through the month of Might 2011 from the region of Konda Thana, Dhaka, Bangladesh. The plants were taxonomically identified and voucher specimen No- DACB 36424 and DACB 36425, have already been maintained for C. cajan and T. indica, respectively, inside the Bangladesh National Herbarium, Dhaka, Bangladesh.Preparation of methanolic extract of C. cajan rootAbout 387 g of dried powdered material was refluxed with 500 ml of 95 methanol for 3 h. The extracts had been then filtered via filter paper (Double Rings filter paper 102, 11.0 cm). The filtrates (methanol extract) have been concentrated at 50 under lessen pressure working with rotary evaporator (STUART RF3022C, UK) to afford an oily concentrate of greenish black colour of extract.Degarelix acetate manufacturer Lastly, we got 16 g methanolic extract. The extract transferred to a closed container for additional use and protection.Preparation of Methanolic extract of T. indica SeedsThe seeds of T. indica have been dried in an oven for 2 days at 40 , crushed in an electrical grinder then powdered.Tomatine In Vitro Extraction was performed by taking 25-g powder in 250 ml of distilled water for 18 h within a soxhlet apparatus in addition to a deepNahar, et al.: Comparison of antidiabetic activity of Cajanus cajan and Tamarindus indicabrown aqueous extract was obtained. The extract was dried at decreased pressure and transferred to a closed container for additional use and protection.PMID:24516446 InstrumentsThe molecular absorption spectra and absorbance at certain wavelengths had been recorded with a HACH DR 4000U UV-visible spectrophotometer equipped with quartz cells of 1-cm light path.Chemical substances and reagentsAll chemical compounds and drugs had been obtained commercially and were of analytical grade. DPPH (Sigma Chemical Co., USA.), Alloxan (Fluka, Germany), ammonium molybdate (Merck, Germany), sodium phosphate (BDH, England), potassium ferricyanide K 3[Fe(CN) 6], trichloroacetic acid (CCl 3 COOH), Folin iocalteu reagent, GA [C6H2(OH)3COOH], ascorbic acid (AA) and metformin hydrochloride (Common Pharmaceutical Bangladesh Ltd) and glucose estimation kit (Human, Germany).In vivo study for assessment of antihyperglycemic activity Experimental animalstudy contains measurement in the blo.