Product Name :
Anti-GFP, AlpSdAbs® VHH(Biotin)
Applications:
ELISA,Purification,BLI,SPR
Reactivity :
GFP
Conjugate:
Biotin
Advantages :
High lot-to-lot consistencyIncreased sensitivity and higher affinityAnimal-free production
Description:
| Description: Anti-GFP, AlpSdAbs® VHH(Biotin) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to GFP coupled to Biotin, and Anti-GFP, AlpSdAbs® VHH(Biotin) detects the GFP selectively, no reactivity with other proteins. | | Immunogen: GFP fusion protein | Host: Alpaca pacous | Isotype: VHH domain of alpaca IgG2b/2c | Conjugate: Biotin | Specificity: GFP | Cross-Reactivity: Recognizes GFP, mEGFP, superfolder GFP and most common CFP and YFP variants. Does not cross-react with mCherry, mRFP, dsRed, mTagBFP, mTagRFP or their most common derivatives. | Purity: Recombinant Expression and Affinity purified | Concentration: 1mg/ml | Buffer: 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300 | Storage: Store at –20 °C(Avoid freeze / thaw cycles)
Description2 :
Anti-GFP, AlpSdAbs® VHH(Biotin) is designed for detecting GFP fusion proteins. Anti-GFP, AlpSdAbs® VHH(Biotin) is based on monoclonal, recombinant, single domain antibody to GFP coupled to Biotin, and Anti-GFP, AlpSdAbs® VHH(Biotin) detects the GFP selectively, no reactivity with other proteins.{{37558-16-0} site|{37558-16-0} Biological Activity|{37558-16-0} Formula|{37558-16-0} manufacturer}
Immunogen:
GFP fusion protein
Host :
Alpaca pacous
Isotype:
VHH domain of alpaca IgG2b/2c
Purity :
Recombinant Expression and Affinity purified
Buffer :
10mM PBS (pH 7.{{2387505-73-7} MedChemExpress|{2387505-73-7} Technical Information|{2387505-73-7} Description|{2387505-73-7} custom synthesis} 5), 0.PMID:25905224 05% sucrose, 0.1% trehalose, 0.01% proclin300
Storage :
Function:
ELISA: 1:5,000-1:20000IP: 1-2ug/sampleBLI (biolayer interferometry)SPR (surface plasmon resonance)Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.
