To investigate the outcomes of AQP9 inhibitors on the development of bleb-like protrusions the cells had been pre-handled for 15 min with one, five or ten mM Hg2+ or twenty five mM HTS13286 (Maybridge, Cornwall, British isles)
Cells have been transfected with tagRFP-AQP9 or vacant vector, collectively with GFP-Mem and seeded...