The other SNPs did not show any reliable association with measures of body fat content and distribution

e Buffered Saline for 15 min at room temperature, washed three times with DPBS and incubated with DPBS containing 10% fetal bovine serum for 30 min at room temperature. The cells were incubated with the primary antibodies for 60 min, and then with the secondary antibodies for 60 min before staining with DAPI. Test of iPS cell viability after UVC irradiation Maxadilan markedly affected cell survival after UVC irradiation. iPS cells that were exposed to 50 J/m2, 75 J/m2 and 100 J/m2 UVC and treated with 30 nM maxadilan showed a significant increase in cell viability compared with iPS cells that were not treated with maxadilan. iPS cells that were irradiated with 50 J/m2, 75 J/m2 and 100 J/m2 UVC showed a 64.63%, 67.23% and 70.8% reduction in cell viability, respectively, compared with the control group. The addition of 30 nM maxadilan resulted in a 51.75%, 53.1% and 52.43% decrease in cell viability, respectively, compared with the control group. iPS cells exposed to 100 J/m2 UVC and treated with 50 nM of maxadilan also showed a significant increase in cell viability compared with iPS cells that were not treated with maxadilan. The viability of iPS cells after irradiation with 100 J/m2 UVC was reduced by 70.8% compared with the control group, and the addition of 50 nM maxadilan displayed a 50.29% decrease compared with the control group. Annexin V and PI assays Annexin V and PI ” were analyzed by flow cytometry to detect apoptosis in iPS cells cultured under various treatments. During the early stages of apoptosis, cells typically have an intact cell membrane that are not buy BioPQQ stained with PI; however, externalization of phosphatidylserine can be detected by annexin V. Using this method, we found that the addition of Statistical Analysis Statistical analysis was performed with a software package. The statistical significance comparing multiple sample sets with 25423286the control was analyzed with a one-way ANOVA followed by the Dunnett’s test. Comparisons between the two 4 Maxadilan Prevents Apoptosis in iPS Cells 30 nM maxadilan to iPS cells irradiated with 100 J/m2 UVC dramatically reduced the ratio of early apoptotic cells compared with iPS cells without maxadilan treatment. iPS cells irradiated with 100 J/m2 UVC showed a 248% increase in the ratio of early apoptotic cells compared with the control group, whereas cells treated with 30 nM maxadilan under the same conditions displayed a 158% increase compared with the control group. cells irradiated with UVC showed a 587% increase in the percentage of apoptotic cells compared with control group, and ” the addition of 30 nM maxadilan displayed only a 224% increase in apoptotic cells compared with the control group. Caspase-3 and caspase-9 assays To analyze the apoptotic machinery of iPS cells induced by UVC and the anti-apoptotic machinery of maxadilan, caspase-3 and caspase-9 assays were performed. Our data showed that the addition of 30 nM maxadilan to iPS cells irradiated with 100 J/m2 UVC significantly downregulated caspase-3 and caspase-9. iPS cells irradiated with 100 J/m2 UVC showed a 104% and 92% increase in activity of the caspase-3 and caspase-9, respectively, compared with the control group, and the addition of 30 nM maxadilan displayed a 51% and 54% increase, respectively, compared with the control group. TUNEL assays A TUNEL assay was performed to assess the anti-apoptotic effects of maxadilan in iPS cells irradiated with UVC. The values of biotinylated fluorescein-dUTP were proportional to