Roved the submission with the manuscript. Acknowledgments The authors are thankful for the Post Graduate College and Director, Indian Agricultural Study Institute, New Delhi, India for delivering for fellowship towards M.Sc. program in the senior author. The authors are also grateful for the Division of Microbiology, Indian Agricultural Investigation Institute (IARI), New Delhi and Indian Council of Agricultural Analysis for providing the facilities and financial assistance, to undertake the investigations. Author particulars 1 Division of Microbiology, Indian Agricultural Study Institute (IARI), New Delhi 110012, India. 2Centre for Lipid Research, CSIR-Indian Institute of Chemical Technologies, Hyderabad 500 007, India. Received: 9 August 2012 Accepted: 28 September 2012 Published: 6 October 2012 References Becker W (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture. Blackwell, Oxford, pp 31251 Behrens PW, Kyle DJ (1996) Microalgae as a supply of fatty acids. J Meals Lipids three:25972 Brennan L, Owende P (2010) Biofuels from microalgae a evaluation of technologies for production, processing, and extractions of biofuels and co-products. Renew Sust Energy Rev 14:55777 Brown ML, Zeiler KG (1993) Aquatic biomass and carbon dioxide trapping. Energy Conv 1-(Anilinocarbonyl)proline Biological Activity Managmt 34:10050013 Bumbak F, Cook S, Zachleder V, Hauser S, Kovar K (2011) Very best practices in heterotrophic high-cell-density microalgal processes: achievements, prospective and probable limitations. Appl Microbiol Biotechnol 91:316 Bus Aust SD, Gibsont JE (1976) Paraquat Toxicity: Proposed Mechanism of Action Involving Lipid Peroxidation. Environ Overall health Perspect 16:13946 Ceron Garcia MC, Sanchez Miron A, Fernandez Sevilla JM, Molina Grima E, Garcia Camacho F (2005) Mixotrophic development from the microalgae Phaeodactylum tricornutum, influence of various nitrogen and organic carbon supply on productivity and biomass composition. Process Biochem 40:3125131 Chen W, Sommerfeld M, Hu Q (2011) Microwave-assisted Nile red technique for in vivo quantification of neutral lipids in microalgae. Bioresour Technol 102:13541 Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:12631 Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:29406 Courchesne NMD, Parisien A, Wang B, Lan CQ (2009) Enhancement of lipid production employing biochemical, genetic and transcription element engineering Phleomycin Technical Information approaches. Biotechnol J 141:31The cells were observed making use of Zeiss Model Axio Scope. A1, after incubation with distinctive substrates. Nile red (9-(Diethylamino) -5H benzo [] phenoxazin- 5-one) staining was employed to detect intracellular lipid droplets (Greenspan et al. 1985).Statistical analysesThe statistical analyses have been performed working with the software program SPSS 10. One-way analysis of variance (ANOVA) was used to evaluate the differences amongst the treatments. In case, the ANOVA effects had been significant, comparisons among the diverse suggests had been created to quantify and evaluate the supply of variation, and CD (Essential Variations) and SEM (Standard error of Implies) values have been calculated at 0.05 P level. SD (Common deviation) values are depicted inside the graphs as error bars. The superscripts in Tables denote the rankings according to Duncan’s Numerous Variety test and unique alphabets denote considerably unique values, as analyzed applying SPSS.Additional filesAdditional file 1: Figure S1. Networking of metabolic processes inside the cell.Ngangkham et al. SpringerPlus 2012, 1:33.
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