Roved the submission with the manuscript. Acknowledgments The authors are thankful towards the Post Graduate School and Director, Indian Agricultural Analysis Institute, New Delhi, India for giving for fellowship towards M.Sc. plan on the senior author. The authors are also grateful to the Division of Microbiology, Indian Agricultural Study Institute (IARI), New Delhi and Indian Council of Agricultural Investigation for giving the facilities and financial help, to undertake the investigations. Author details 1 Division of Microbiology, Indian Agricultural Analysis Institute (IARI), New Delhi 110012, India. 2Centre for Lipid Research, CSIR-Indian Institute of Chemical Technologies, Hyderabad 500 007, India. Received: 9 August 2012 Accepted: 28 September 2012 Published: six October 2012 References Becker W (2004) Microalgae in human and animal nutrition. In: Richmond A (ed) Handbook of microalgal culture. Blackwell, Oxford, pp 31251 Behrens PW, Kyle DJ (1996) Microalgae as a supply of fatty acids. J Food Lipids 3:25972 Brennan L, Owende P (2010) Biofuels from microalgae a overview of technologies for production, processing, and extractions of biofuels and co-products. Renew Sust Power Rev 14:55777 Brown ML, Zeiler KG (1993) Aquatic biomass and carbon dioxide trapping. Energy Conv Managmt 34:10050013 Bumbak F, Cook S, Zachleder V, Hauser S, Kovar K (2011) Finest practices in heterotrophic high-cell-density microalgal processes: achievements, prospective and possible limitations. Appl Microbiol Biotechnol 91:316 Bus Aust SD, Gibsont JE (1976) 5-Acetylsalicylic acid Technical Information Paraquat Toxicity: Proposed Mechanism of Action Involving Lipid Peroxidation. Environ Health Perspect 16:13946 Ceron Garcia MC, Sanchez Miron A, Fernandez Sevilla JM, Molina Grima E, Garcia Camacho F (2005) Mixotrophic growth on the microalgae Phaeodactylum tricornutum, influence of diverse nitrogen and organic carbon source on productivity and biomass composition. Process Biochem 40:3125131 Chen W, Sommerfeld M, Hu Q (2011) Microwave-assisted Nile red technique for in vivo quantification of neutral lipids in microalgae. Bioresour Technol 102:13541 Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:12631 Chisti Y (2007) Biodiesel from microalgae. Biotechnol Adv 25:29406 Courchesne NMD, Parisien A, Wang B, Lan CQ (2009) Enhancement of lipid production working with biochemical, genetic and transcription aspect engineering approaches. Biotechnol J 141:31The cells have been observed utilizing Zeiss Model Axio Scope. A1, immediately after incubation with distinctive substrates. Nile red (9-(Diethylamino) -5H benzo [] phenoxazin- 5-one) staining was utilized to detect intracellular lipid droplets (Greenspan et al. 1985).Statistical analysesThe statistical analyses had been performed utilizing the LP-922056 Biological Activity application SPSS ten. One-way evaluation of variance (ANOVA) was applied to evaluate the variations amongst the remedies. In case, the ANOVA effects had been significant, comparisons amongst the distinct signifies have been created to quantify and evaluate the supply of variation, and CD (Critical Variations) and SEM (Regular error of Suggests) values have been calculated at 0.05 P level. SD (Common deviation) values are depicted in the graphs as error bars. The superscripts in Tables denote the rankings determined by Duncan’s Many Variety test and various alphabets denote drastically distinctive values, as analyzed employing SPSS.Extra filesAdditional file 1: Figure S1. Networking of metabolic processes inside the cell.Ngangkham et al. SpringerPlus 2012, 1:33.
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