Nter for Algorithmic Biotechnology, St. Petersburg State University, 199004 Saint-Petersburg, Russia; a.Nter for Algorithmic Biotechnology,

Nter for Algorithmic Biotechnology, St. Petersburg State University, 199004 Saint-Petersburg, Russia; a.
Nter for Algorithmic Biotechnology, St. Petersburg State University, 199004 Saint-Petersburg, Russia; [email protected] Division of Phytotoxicology and Biotechnology, All-Russian Institute of Plant Protection, 196608 Saint-Petersburg, Russia; [email protected] Correspondence: [email protected]: Rayko, M.; Sokornova, S.; Lapidus, A. Fungal Metagenome of Chernevaya Taiga Soils: Taxonomic Composition, Differential Abundance and Factors Related to Plant Gigantism. J. Fungi 2021, 7, 908. https://doi.org/10.3390/jof7110908 Academic Editor: Kuang R. Chung Received: 12 September 2021 Accepted: 23 October 2021 Published: 27 OctoberAbstract: The Chernevaya taiga of Western Siberia is really a special and complicated ecosystem, distinguished by the unusually substantial sizes of herbaceous plants, the factors for which are poorly understood. Right here, we explored the fungal diversity in the Chernevaya taiga soils within the Tomsk regions of Western Siberia in comparison with other soil sorts. The soil biomes of Chernevaya taiga as well as the control regions were investigated utilizing Illumina ITS rRNA sequencing, and taxonomic analysis revealed a predominance of fungal phyla in the unique soils. These benefits demonstrate that the fungi of the Chernevaya taiga regions possess a larger species diversity (Faith’s PD) vs. the handle soils, and the diversity is due much more to the sampling sites as opposed to for the seasons (Bray-Curtis distance). We studied a lot of the differentially abundant taxa among the soil types, and we annotated the taxa with their ecological guilds and trophic types. A few of the abundant fungal taxa in the summer- and fall-Chernevaya taiga samples belong to the phylum Glomeromycota–arbuscular Scaffold Library Solution mycorrhizal symbiotrophs, which are recognized to establish symbiotic relationships and enhance plant growth. Also, various OTUs were assigned to novel genera within the Glomeraceae and Claroideoglomeraceae households. Our findings add a potential explanation on the high productivity and plant gigantism in Chernevaya taiga and expand our expertise of fungal biodiversity. Keywords: fungi; diversity; neighborhood composition; plant gigantism; arbuscular mycorrhiza; Chernevaya taiga; soil properties; FUNGuild1. Introduction The forests of Chernevaya taiga represent unique boreal forest formations which are limited in their distribution by hyper-humid sectors in the Altai-Sayan mountain region. These ecosystems are characterized by many attributes: (i) the growth of two major dominant tree species–Siberian fir (Abies sibirica) and widespread aspen (Populus tremula); (ii) tall-growing shrubs for instance Siberian mountain-ash (Sorbus sibirica), birds cherry (Padus avium), and goat willow (Salix caprea) that can reach as much as 18 m in height and 30 cm in diameter; (iii) dense layers of massive perennial herbs of as much as 4.5 m; (iv) many species representative of Tertiary nemoral relict flora; (v) an absence of moss cover around the soil surface [1]. The soils of Chernevaya taiga represent some of essentially the most exciting but not but studied subjects of metagenomic analysis, given that they demonstrate a number of unusual Goralatide Protocol attributes, including extremely tall grasses with an overall low concentration of humus (efficient fertility), and an unusually higher decomposition rate of plant residues. Obviously, both the productivity of Chernevaya taiga soils and also the rapid decomposition of leaf litter are a complex of associated properties, the analysis of which inside the framework of a metagenomic study is definitely an crucial fundam.