Or automobile. In either case C60 exposure exacerbated myocardial infarction. Within every delivery route, infarct

Or automobile. In either case C60 exposure exacerbated myocardial infarction. Within every delivery route, infarct sizes within the male groups were larger than those in females. Amongst delivery routes, the β-lactam Inhibitor Species females had bigger infarctions in response to IT C60 exposure compared with IV exposure. p 0.05 by two-way ANOVA, N = four?.α adrenergic receptor Agonist MedChemExpress exposed to IV C60 when compared with serum collected from male rats exposed to IV automobile and male rats exposed to IT C60 (Fig. 4A). Female rats exposed to IV C60 had significantly reduce serum IL-6 concentrations than IV C60 exposed males. Serum IL-6 concentrations had been unaltered between all IT groups and IV exposed females. MCP-1 showed a equivalent profile to IL-6. MCP-1 concentrations have been greater in serum-collected post-I/R from male rats exposed to C60 IV when compared with serum collected from male rats exposed to IV automobile, male rats exposed to IT C60 and female rats exposed to C60 IV (Fig. 4B). Female rats exposed to IV C60 had drastically lower serum MCP1 concentrations than IV C60 exposed males. Serum MCP-1 concentrations have been unaltered amongst all IT groups and IV exposed females. VEGF was variable and slightly elevated in females exposed to IT and IV C60 , even though not considerably dif-TABLE 2 Rat Pulmonary Responses to Intratracheal (IT) or Intravenous (IV) Delivery of C60 or Automobile SuspensionsTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in males, data expressed as mean ?SEM Protein ( g/ml) IT Automobile 560 ?149 C60 316 ?55 Na�ve i 456 ?31 n 4? p 0.05 versus IT C60. IV 638 ?one hundred 750 ?138 Cell number (?105 ) IT five.7 ?1.4 three.9 ?0.eight five.6 ?0.five 4? IV three.5 ?0.9 6.9 ?1.7 Macrophages (?105 ) IT 5.3 ?1.three four.0 ?0.7 5.6 ?0.5 three? IV five.7 ?0.7 2.5 ?1.two Neutrophils (x 103 ) IT 14.2 ?10 13.three ?12 7.7 ?4.two three? IV two.1 ?0.three 1.7 ?1.four Eosinophils (?03 ) IT 1.9 ?1.1 0.five ?0.five 1 three? IV 2.four ?two.4 1 Epithelial cells (?103 ) IT six.six ?two.4 two.8 ?0.3 8.7 ?four.0 3? IV 1.7 ?1.7 8.two ?2.6 CARDIOVASCULAR INJURY IN RESPONSE TO CTotal bronchoalveolar lavage fluid (BALF) protein and cellularity BAL samples collected 24 h following exposure in females, information expressed as imply ?SEM Protein ( g/ml) Automobile C60 Na�ve i n IT 252 ?28 206 ?24 IV 195 ?eight.2 226 ?41 N/A 5? Cell quantity (?105 ) IT IV 7.8 ?1.9 four.1 ?0.6 7.1 ?1.two three.5 ?0.five six.six ?1.0 4? Macrophages (?105 ) IT IV 1.6 ?0.9 three.3 ?0.4 0.9 ?0.three 3.1 ?1.1 six.6 ?1.1, 4? Neutrophils (?103 ) IT 35.0 ?25.3 27.5 ?11.1 IV 1 1 Eosinophils (?103 ) IT IV 0.25 ?0.25 1.7 ?1.7 12.five ?7.5 three.6 ?0.six 1 4? Epithelial cells (?103 ) IT IV 132 ?90 346 ?132 75.0 ?33 362 ?59 179 ?43 4?1 four?N/A, not applicable. p 0.05 versus IT C60 . p 0.05 versus IT automobile.THOMPSON ET AL.TABLE three Rat Coronary Artery Pharmacological Responses for Serotonin (5-HT), ACh, SNP, and ET-Male rat EC50 s [nM] (mean ?SEM) IT delivery C60 5-HT ACh SNP ET-1 559 ?104 265 ?45 116 ?24 38 ?20 Vehicle 871 ?130 239 ?34 76 ?18 48 ?15 IT delivery 5-HT ACh SNP ET-1 C60 1.7 ?0.1 2.6 ?0.4 two.four ?0.6 1.three ?0.2 Automobile 3.2 ?0.7 1.7 ?0.1 2.2 ?0.three 1.0 ?0.05 IT delivery 5-HT ACh SNP ET-1 C60 813 ?128 1419 ?843 19 ?3 29 ?20 Automobile 1268 ?497 139 ?28 21 ?4 6.5 ?two.4 IT delivery 5-HT ACh SNP ET-1 C60 1.4 ?0.4 1.0 ?0.four 1.6 ?0.3 1.4 ?0.three Vehicle 1.0 ?0.two 1.six ?0.3 two.1 ?0.4 1.1 ?0.three p-value, n 0.39, 5 0.29, five 0.35, 4 0.41, 5 C60 1.3 ?0.4 1.0 ?0.2 two.0 ?0.3 1.1 ?0.two p-value, n C60 0.ten, four 2.0 ?0.2 0.10, four three.eight ?0.8 0.77, 4 two.1 ?0.three 0.09, 6 1.1 ?0.1 Female rat EC50 s [nM] (imply ?SEM) p-value, n 0.06, 4 0.33, four 0.13, four 0.49, 6 C60 IV deliv.