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[pSer71]Vimentin monoclonal antibody (TM71)
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Vimentin is one of four type II intermediate filament (IF) proteins; Desmin, Glial fibrillary acidic protein (GFAP) and Peripherin being the others. Vimentin is the most widely distributed of all IF proteins. It is typically expressed in leukocytes, blood vessel endothelial cells, and mesenchymal cells such as fibroblasts. Vimentin filaments help support cellular membranes. Vimentin networks also may help keep the nucleus and other organelles in a defined place within the cell. Western blot analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71). Immunocytochemistry analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71). Western blot analysis of phosphorylated vimentin (Ser71) expression in U251 using [pSer71]Vimentin, mAb (TM71). Immunocytochemistry analysis of phosphorylated vimentin (Ser71) on 4% PFA fixed U251 cells with [pSer71]Vimentin, mAb (TM71).
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| Alternative Name VIM | Application ICC, WB | Application Notes Detects a band of ~58kDa by Western blot.{{4434-05-3} MedChemExpress|{4434-05-3} Protocol|{4434-05-3} Data Sheet|{4434-05-3} custom synthesis} | Clone TM71 | Formulation Liquid.{{2108782-45-0} web|{2108782-45-0} Purity & Documentation|{2108782-45-0} Formula|{2108782-45-0} supplier} In PBS, pH 7.PMID:30137828 2, containing 50% glycerol. | Host Rat | Immunogen Synthetic peptide corresponding to aa 66-77 of human Vimentin phosphorylated at Ser71. | Isotype IgG2a | Recommendation Dilutions/Conditions Immunocytochemistry (1µg/ml)Western Blot (1µg/ml for chemiluminescence detection system)Suggested dilutions/conditions may not be available for all applications.Optimal conditions must be determined individually for each application. | Species Reactivity Human, Mouse, Rat | UniProt ID P08670 | Unit of Measure (UM) µg
