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In the most cancers cells that exist in this kind of environments, the monitoring of and adaptation to extracellular glucose concentrations are assumed to be important for the survival/proliferation of the tumor cells. We earlier documented that AKT phosphorylation

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Opposite to the traditional system, protein expression is induced in the vicinity of saturation...
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The purity of the GST-fusion proteins was assayed by SDS-Web page. GST-tagged NT-, spectrin-, onco- and PH- Dbl mutant constructs, described previously in [31], have been expressed in COS1 cells, lysed with HEPES buffer as in [31] and purified with glutathione-sepharose

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Protein expression was confirmed by western blot analysis. All experiments had been completed with...
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IFNc was ordered from BD Biosciences (San Jose, CA).Generation of Caenorhabditis elegans luciferase- and mouse protein kinase D1 gene (Prkd1)-knockdown macrophages working with a vector expressing a gene-precise tiny hairpin interfering (sh) RNA

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These defects are obvious contributors to the reduced survival prospective of homozygotes, and emphasize...
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For detection of GFP-CTCF and GFP-BORIS fusion proteins, transfected HEK293T cells have been preset with chilly methanol (0uC), washed and mounted with anti-fading mounting medium Vectashield (Vector Laboratories) with forty nine,69Diamidino-2-phenylindole dihydrochloride

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In order to get insight into this challenge we have analyzed a main human...